Journal: Scientific Reports

Article Title: Single ingestion of soy β-conglycinin induces increased postprandial circulating FGF21 levels exerting beneficial health effects

doi: 10.1038/srep28183

Figure Lengend Snippet: Five-week old male mice were acclimated to the casein high-fat diet for 3 days. After 24 h fasting, mice were fed with either the casein or β-conglycinin (β-con) high-fat diet for 6 h as shown in . Using liver total RNA, microarray analysis was performed according to the manufacturer’s instructions (n = 4). ( a ) GO terms associated with the genes that were up-regulated in the β-conglycinin group. FDR-corrected P values were defined by the modified Fisher’s exact test with the Benjamini and Hochberg FDR correction. FDR-corrected P values < 0.05 are shaded in gray. ( b ) GO terms associated with the genes that were down-regulated in the β-conglycinin group. ( c ) Heatmap shows top twenty genes up-regulated or down-regulated by β-conglycinin ingestion from DNA microarray experiments. Green color indicates upregulation and red indicates downregulation in response to ingestion of two types of diet. ( d ) RT-qPCR was performed using total RNA used in DNA microarray experiments (n = 5). Relative expression levels of FGF21 and ATF4 mRNA in the liver are normalized to cyclophilin mRNA levels and are shown as fold induction to expression levels in the casein group. Serum FGF21 concentrations are determined by ELISA. All data are expressed as means ± SD (n = 5). ** P < 0.01 determined by two-tailed Student’s t -test.

Article Snippet: Serum insulin concentrations were measured using Lbis ® Insulin-mouse ELISA Kit (T type) (SHIBAYAGI Co. Ltd.).

Techniques: Microarray, Modification, Quantitative RT-PCR, Expressing, Enzyme-linked Immunosorbent Assay, Two Tailed Test